Cipa Assay Test

The Cipa Assay Test is a recognized, widely used medical laboratory test that specifically checks and measures cardiac disease. It is designed to evaluate and monitor blood vessel health and functionality in patients suspected of having either a mild or severe heart condition. This method has proven effective in the detection and treatment of patients with cardiomyopathy (an abnormal heart rhythm) and heart valve diseases such as mitral valve prolapse, congenital heart defects, heart muscle disease, congenital heart disease, and heart muscle disease caused by congenital abnormalities at the level of the heart muscles themselves. Furthermore, the Cipa also monitors for abnormalities at the level of the heart muscle itself, particularly at the mitral valve prolapse. These tests have been approved by the FDA.

How Does This Process Occur?

The Cipa uses two different methods to measure cardiac function. One method called the intracerebroventricular (ICV) test assesses the electrically activated calcium spikes in blood samples. This type of test measures the activity of the ion channels along the cellular membrane of the heart. The other method, called the echocardiogram, measures the electric fields of the heart at rest as well as during exercise, and these fields are measured to determine the contractility and motion of the heart. The Cipa assay from Clyde biosciences uses a variety of electrophysiology techniques to assess the health of the heart and its components.

The Cipa utilizes four different types of probes based on distinct functional groups of the ion channels as described above. The first two categories are termed “functionalizing agents” and “monoaminant compounds”. In the functionalizing agents, the nanion substrates with the specific nanomolecules are tested for their ability to activate the cardiac ion channels. There are four different classes in which these substances are placed: non-nucleoside, nucleotide, ATP-responsive component and non-ATP-responsive component.

Research Protocol And Concepts

The primary research of the Cipa laboratory includes work performed in conjunction with Cardiovascular Research and Development Institute (CRD Institute). There has been ongoing studies since the late 1980s utilizing cardiac imaging to demonstrate that in patients with cardiomyopathy, protein deposits in the myocardium cause abnormal electrical responses within the cardiac cells. From this work came the concept of testing the effects of nanion substrates on the electrical firing of the cells. Using an initial technique known as the “patch clamping”, the researchers utilized fluorescent dye to visualize the changes in electrical firing when the nanion substrates were applied across the patch.

The basic procedure for patch clamping is not too dissimilar to that of other medical imaging procedures used in the laboratory. A light emitting diode (LED) is attached to the patch of tissue with the purpose of activating the dye. Once this is done, it is gently tugged by a flexible probe called a “Patch Clamp” to expose the dye to the region of concern. This allows the scientist to determine the intensity and location of the calcium uptake change. The same technology is employed in the Cipa test. However, the difference lies in the fact that the patch clamp is utilized in a non-invasive manner without any invasive equipment or instruments.


As indicated previously, the Cipa assay test is currently undergoing several animal studies. These studies will utilize genetically engineered animals to determine whether this technology is both safe and efficient in humans. There are currently three animal models designated for the Cipa syllabus, namely, Synchronized Multicystic (SMC), Atripla (AR), and Cipa HEV (chest). The exact studies involving these animals will be conducted according to the current CIPA assay protocol.